Fibrin scaffold fits as a provisional platform promoting cell migration and proliferation,\nangiogenesis, connective tissue formation and growth factors stimulation. We evaluated a unique\nheterologous fibrin biopolymer as scaffold to mesenchymal stem cells (MSCs) to treat a critical-size\nbone defect. Femurs of 27 rats were treated with fibrin biopolymer (FBP); FBP + MSCs; and FBP\n+ MSC differentiated in bone lineage (MSC-D). Bone repair was evaluated 03, 21 and 42 days later\nby radiographic, histological and scanning electron microscopy (SEM) imaging. The FBP + MSC-D\nassociation was the most effective treatment, since newly formed Bone was more abundant and early\nmatured in just 21 days. We concluded that FBP is an excellent scaffold for MSCs and also use of\ndifferentiated cells should be encouraged in regenerative therapy researches. The FBP ability to\nmaintain viable MSCs at Bone defect site has modified inflammatory environment and accelerating\ntheir regeneration.
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